Massimiliano Allegri is hoping to finish third in Serie A after beating Juventus 2-1 with Sassuolo.
The Bianconeri took a big step towards qualifying for the Champions League on Monday after Giacomo Raspadori opened the scoring for all three points at MAPEI Stadium - Citta del Tricolore.
Paulo Dybala equalized in the 88th minute and Moise Kean came off the bench to win in the 88th minute.
Juventus have won four of their last four games, eight ahead of fifth-placed Roma and just one point behind third-placed Napoli.
Dassico Berardi, who has scored 12 goals in Serie A, and Sassuolo, who posed a serious threat to go ahead, suffered the same defeat in the same season since Ronaldinho, who was the most threatened player in Serie A since 2009-10.
At the start of the second half, Dusan Vlahovic and Giorgio Chiellini were brought in, but Juventus coach Allegri, who left Matthijs de Ligt on the bench due to illness, said it was "impossible" to miss his players for the rest of the season.
"We are one point ahead of Napoli. We hoped to play well and get through a lot of positive results, so we tried to get closer to the leaders but failed.
"I have to do my best from now until the end."
Juventus had a difficult start to the season, but the Allegri was pleased with the pace they took early in their second game against the club, who have won just 19 of their last 19 Serie A games this season, beating Inter Milan by just 19 points. I earn more.
I think Juventus has laid a good foundation for the future and there is room for improvement. There are also those who arrived in January.
"I think Juventus need to understand that they are working in a specific way and have a good foundation.
"We know how we work every day, we know inside out, we try to do a good job every day, sometimes the seasons are born one way or another, the end is over, there are situations like injuries, now there are five of us.
"[Federico] Chiesa has been out for a long time and [Weston] McKennie is the same. They have the necessary characteristics. I think the team is doing well. The team is adaptable and doing well.